Abstract

Nitric oxide (NO) is considered to be involved in the modulation of uterine contractility.

In the present pilot study, the direct detection of intracellular NO in pregnant human myometrial tissues has been investigated by using the fluorescent indicator 4,5-diaminofluorescein-2 diacetate (DAF-2DA).

Pregnant myometrial tissue samples were obtained during Cesarean sections between week 34 and 40 of gestation before the onset of labor.

Living explants were loaded with 10 μM DAF-2DA, stimulated with 1 mM arginine, subsequently fixed with glutaraldehyde and examined ...

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